![]() Large scale production of erythritol is currently based on conversion of glucose by selected fungi. Due to its non-caloric and non-cariogenic properties, the popularity of this sweetener is increasing. Van der Woude, Aniek D Perez Gallego, Ruth Vreugdenhil, Angie Puthan Veetil, Vinod Chroumpi, Tania Hellingwerf, Klaas JĮrythritol is a polyol that is used in the food and beverage industry. Genetic engineering of Synechocystis PCC6803 for the photoautotrophic production of the sweetener erythritol. ![]() Hellingwerf, K.J.īACKGROUND: Erythritol is a polyol that is used in the food and beverage industry. Genetic engineering of Synechocystis PCC6803 for the photoautotrophic production of the sweetener erythritol The optimum medium composition for erythritol production was found to be (g/l) glucose 279.49, ammonium sulfate 9.28, and pH 5.41 with 39.76 erythritol production. In order to enhance erythritol production, we used a three component-three level-one response Box-Behnken of response surface methodology model. A general comparison of wild and mutant gene sequences showed the replacement of Asp(270) with Glu(270) in ER protein. The ER gene of wild and mutant strains was sequenced and analyzed. Erythritol production and specific ER activity in mutant 49 increased to 1.65 and 1.47 times, respectively, in comparison with wild-type strain. One of the mutants named as mutant 49 gave maximum erythritol production without any other by- products (particularly glycerol). Productivity of samples was analyzed by thin-layer chromatography and high-performance liquid chromatography equipped with the refractive index detector. The mutants showing the highest ER activity were screened by triphenyl tetrazolium chloride agar plate assay. lipolytica were generated by ultra-violet for enhancing erythrose reductase (ER) activity and erythritol production. The purpose of the present investigation was to produce erythritol by Yarrowia lipolytica mutant without any by- products. Ghezelbash, Gholam Reza Nahvi, Iraj Emamzadeh, Rahman Improvement of erythrose reductase activity, deletion of by- products and statistical media optimization for enhanced erythritol production from Yarrowia lipolytica mutant 49.
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